https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37385 Wed 28 Oct 2020 18:12:02 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52876 Wed 28 Feb 2024 11:01:07 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45249 Wed 26 Oct 2022 19:43:42 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45260 Wed 26 Oct 2022 18:02:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45204 Wed 26 Oct 2022 15:29:09 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40952 Wed 20 Jul 2022 15:22:13 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49349 Wed 17 Apr 2024 10:02:24 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40283 Wed 13 Mar 2024 08:47:04 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15653 Wed 11 Apr 2018 17:40:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1527 Wed 11 Apr 2018 16:54:55 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14485 Wed 11 Apr 2018 16:08:44 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4505 Wed 11 Apr 2018 16:03:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:16821 Wed 11 Apr 2018 15:45:52 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15122 Wed 11 Apr 2018 15:13:29 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15133 Wed 11 Apr 2018 14:23:25 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27762 in vitro fertilization (IVF) success. Furthermore, reduced expression of HSPA2 from the human sperm proteome leads to an impaired capacity for cumulus matrix dispersal, sperm-egg recognition and fertilization following both IVF and ICSI. In this review, we consider the evidence supporting the role of HSPA2 in sperm function and explore the potential mechanisms by which it is depleted in the spermatozoa of infertile patients. Such information offers novel insights into the molecular mechanisms governing sperm function.]]> Wed 11 Apr 2018 13:54:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26480 Wed 11 Apr 2018 13:21:00 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30804 Wed 11 Apr 2018 12:10:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27712 Wed 11 Apr 2018 12:01:24 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27739 Wed 11 Apr 2018 11:56:44 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30207 350 microRNAs (miRNAs), a developmentally important sRNA class, the majority (~60%) of which are also represented by the miRNA signature of spermatozoa. This includes >50 miRNAs that were found exclusively in epididymal sperm and epididymosomes, but not in the surrounding soma. We also documented substantial changes in the epididymosome miRNA cargo, including significant fold changes in almost half of the miRNAs along the length of the epididymis. Finally, we provide the first direct evidence for the transfer of several prominent miRNA species between mouse epididymosomes and spermatozoa to afford novel insight into a mechanism of intercellular communication by which the sRNA payload of sperm can be selectively modified during their post-testicular maturation.]]> Wed 11 Apr 2018 11:13:04 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4506 Wed 11 Apr 2018 10:11:11 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37820 Wed 09 Feb 2022 15:58:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38419 Wed 08 Sep 2021 16:10:39 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44031 5-years following diagnosis, however, for those that fail upfront therapies, survival is poor. Reactive oxygen species (ROS) are elevated in a range of cancers and are emerging as significant contributors to the leukaemogenesis of ALL. ROS modulate the function of signalling proteins through oxidation of cysteine residues, as well as promote genomic instability by damaging DNA, to promote chemotherapy resistance. Current therapeutic approaches exploit the pro-oxidant intracellular environment of malignant B and T lympho-blasts to cause irreversible DNA damage and cell death, however these strategies impact normal haematopoiesis and lead to long lasting side-effects. Therapies suppressing ROS production, espe-cially those targeting ROS producing enzymes such as the NADPH oxidases (NOXs), are emerging alternatives to treat cancers and may be exploited to improve the ALL treatment. Here, we discuss the roles that ROS play in normal haematopoiesis and in ALL. We explore the molecular mechanisms underpinning overproduction of ROS in ALL, and their roles in disease progression and drug resistance. Finally, we examine strategies to target ROS production, with a specific focus on the NOX enzymes, to improve the treatment of ALL.]]> Wed 05 Oct 2022 15:18:52 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43968 Wed 05 Oct 2022 14:15:31 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38138 Wed 04 Aug 2021 15:42:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45607 Wed 02 Nov 2022 14:14:14 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47818 Wed 01 Feb 2023 09:51:54 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49743 Tue 30 May 2023 15:29:49 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47613 Tue 24 Jan 2023 11:43:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51137 Tue 22 Aug 2023 15:58:50 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51128 6,000 proteins, encompassing the selective loss and gain of several hundred proteins. Further, we demonstrate epididymal-driven activation of RHOA-mediated signaling pathways is an important component of sperm maturation. These data contribute molecular insights into the complexity of proteomic changes associated with epididymal sperm maturation.]]> Tue 22 Aug 2023 15:52:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35957 Tue 21 Jan 2020 11:08:08 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39108 Tue 10 May 2022 10:14:50 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39106 Tue 10 May 2022 09:39:30 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35834 Kifc1 and Kifc5b, in aged oocytes; family members selectively targeted for expression regulation by endo-siRNAs of elevated abundance. The implications of reduced Kifc1 and Kifc5b expression were explored using complementary siRNA-mediated knockdown and pharmacological inhibition strategies, both of which led to increased rates of aneuploidy in otherwise healthy young oocytes. Collectively, our data raise the prospect that altered sRNA abundance, specifically endo-siRNA abundance, could influence the quality of the aged oocyte.]]> Tue 10 Dec 2019 16:34:23 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36571 Tue 09 Jun 2020 11:40:42 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39495 Crocodylus porosus as a tractable model for understanding crocodilian sperm physiology. In extending our systematic characterisation of saltwater crocodile spermatozoa, in this study we examined the development of motility during sperm transport through the excurrent duct system of the male crocodile. The results show that approximately 20% of crocodile testicular spermatozoa are immediately motile but experience a gradient of increasing motility (percentage motile and rate of movement) as they transit the male reproductive tract (epididymis). Moreover, we confirmed that, as in ejaculated crocodile spermatozoa, increased intracellular cAMP levels promoted a significant and sustained enhancement of sperm motility regardless of whether the cells were isolated from the testis or epididymis. Along with the development of artificial reproductive technologies, this research paves the way for the opportunistic recovery, storage and potential utilisation of post-mortem spermatozoa from genetically valuable animals.]]> Tue 09 Aug 2022 14:34:34 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40953 Tue 07 Nov 2023 09:51:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33498 Tue 06 Nov 2018 11:19:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43823 Tue 04 Oct 2022 11:04:24 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34727 Tue 03 Sep 2019 17:57:47 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34948 Tue 03 Sep 2019 17:57:02 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34015 Tue 03 Sep 2019 17:54:59 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34016 Thu 30 May 2019 15:47:52 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34014 Thu 30 May 2019 15:41:20 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34868 Thu 30 May 2019 15:31:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49642 Thu 25 May 2023 08:40:46 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:465 Thu 25 Jul 2013 09:09:55 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43344 Thu 15 Sep 2022 15:19:01 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43294 Thu 15 Sep 2022 12:51:41 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35956 Thu 14 May 2020 15:11:42 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1255 Thu 09 Nov 2023 15:42:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47101 Thu 06 Jul 2023 14:06:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30490 Thu 03 Feb 2022 12:19:24 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1202 Thu 01 Aug 2019 17:27:56 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8464 Sat 24 Mar 2018 08:42:09 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7212 Sat 24 Mar 2018 08:39:56 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7044 Sat 24 Mar 2018 08:37:51 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9651 Sat 24 Mar 2018 08:35:26 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1978 region 7 > region 6 > region 1 > region 8 > ductuli efferentes), and orchidectomy had little effect on the rates. Incorporation into four proteins that were secreted in vitro (Mr 38 000, 20 000, 15 000, and 13 000) was reduced or abolished by orchidectomy and restored by testosterone therapy. The secretion of three proteins (Mr 52 000, 23 000, and 22 000) was reduced or abolished by orchidectomy and not restored by testosterone therapy. SDS-PAGE of detergent extracts of sperm indicated that five proteins were lost and nine were gained during epididymal transit. Seven of the proteins gained were about the same molecular weight as proteins secreted by the epididymis (Mr 94 000, 52 000, 38 000, 36 000, 22 000, 20 000, and 13 000) and were analyzed using N-terminal amino acid microsequencing.]]> Sat 24 Mar 2018 08:33:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13040 Sat 24 Mar 2018 08:16:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10106 Sat 24 Mar 2018 08:07:16 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17414 Sat 24 Mar 2018 08:01:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20252 Sat 24 Mar 2018 07:59:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17663 Sat 24 Mar 2018 07:57:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17664 Sat 24 Mar 2018 07:57:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20323 Sat 24 Mar 2018 07:55:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21362 Sat 24 Mar 2018 07:51:24 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5001 Sat 24 Mar 2018 07:44:13 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5003 Sat 24 Mar 2018 07:44:13 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29633 2+2+ release and the suite of molecular modifications that sweep through the oocyte to ensure the successful union of the parental germlines and ensure embryogenic fidelity.]]> Sat 24 Mar 2018 07:41:53 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27766 Sat 24 Mar 2018 07:40:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27768 Sat 24 Mar 2018 07:40:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27976 50 = 6.7 nM) led to a significant reduction in dynamin phosphorylation (10.3% vs. 27.3%; P < 0.001), acrosomal exocytosis (9.7% vs. 25.7%; P < 0.01), and in vitro fertilization (53% vs. 100%; P < 0.01). GSK3 was shown to be present in developing germ cells where it colocalized with dynamin in the peri-acrosomal domain. However, additional GSK3 was acquired by maturing mouse spermatozoa within the male reproductive tract, via a novel mechanism involving direct interaction of sperm heads with extracellular structures known as epididymal dense bodies. These data reveal a novel mode for the cellular acquisition of a protein kinase and identify a key role for GSK3 in the regulation of sperm maturation and acrosomal exocytosis.—Reid, A. T., Anderson, A. L., Roman, S. D., McLaughlin, E. A., McCluskey, A., Robinson, P. J., Aitken, R. J., Nixon, B. Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation.]]> Sat 24 Mar 2018 07:38:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26158 Sat 24 Mar 2018 07:35:27 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27718 via the enzyme, cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1). Acrylamide was administered to male CD1 mice for three or sixmonths at a dose of 0.18 mg/kg bodyweight/day. DNA damage was detected in germ cells and maturespermatozoa of exposed mice without compromising their overall fertility. The use of resveratrol, anantioxidant and known CYP2E1 inhibitor, was found to ameliorate the DNA damage in both germ cells andspermatozoa. However, extended resveratrol treatment (six months, 10.0 mg/kg bw/week) resulted inpremature activation of these cells. Thus the DNA damage found in spermatozoa after chronic acrylamideadministration can be alleviated but an alternative CYP2E1 inhibitor may be required.]]> Sat 24 Mar 2018 07:24:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26537 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.]]> Sat 24 Mar 2018 07:23:27 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22263 in vitro induction of acrosomal exocytosis by progesterone, but not by the calcium ionophore A23187, and elicited a concomitant reduction of in vitro fertilization. In vivo treatment with these inhibitors also resulted in spermatozoa displaying reduced acrosome reaction potential. Dynamin 1 and 2 phosphorylation increased on progesterone treatment, and this was also selectively blocked by dynasore. On the basis of our collective data, we propose that dynamin could regulate specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.]]> Sat 24 Mar 2018 07:17:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25108 Sat 24 Mar 2018 07:17:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25107 Sat 24 Mar 2018 07:17:06 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24845 Sat 24 Mar 2018 07:11:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49719 Mon 29 May 2023 15:14:02 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51259 Mon 28 Aug 2023 13:50:03 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37797 Mon 26 Apr 2021 12:23:37 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32871 in vitro fertilisation, though displayed reduced developmental competence beyond the 2-cell stage (P=0.02). To account for these findings, we analysed the efficacy of DSB resolution, revealing a significant reduction in DSB lesions 4 h post-ETP treatment. Notably, this response was completely abrogated by pharmacological inhibition of key elements (DNA-PKcs and DNA ligase IV) of the canonical non-homologous end joining DNA repair pathway, thus providing the first evidence implicating this reparative cascade in the protection of the maternal genome.]]> Mon 23 Sep 2019 10:15:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54399 Mon 18 Mar 2024 13:06:51 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40758 Mon 18 Jul 2022 13:54:17 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34117 Mon 11 Feb 2019 16:05:21 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33479 Mon 05 Nov 2018 11:10:42 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48239 Mon 01 May 2023 14:49:55 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41288 Mon 01 Aug 2022 11:41:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40281 Fri 29 Jul 2022 13:23:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51228 Fri 25 Aug 2023 11:51:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41982 Fri 19 Apr 2024 11:58:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49362 Fri 12 May 2023 12:35:13 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37679 Fri 12 Mar 2021 10:57:58 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55287 Fri 10 May 2024 16:22:11 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48188 Fri 07 Jul 2023 11:41:15 AEST ]]>